A process for the production of anti-listerial bacteriocin

the present invention deals with a process for producing anti-listerial bacteriocin using Enterococcus faecium (MTCC 5153) up to a yield of 150X103AUml"1. The process comprises synthesis of galactose by hydrolysis of lactose without adding any external sugar, hence cost effective and less time consuming. At least up to 50% of bacteriocin being produced in the first 10 hours of the process. The said bacteriocin is useful for enhancement of lactose intolerant food grade and shows inhibition to Listeria monocytogenes at least up to 3 folds in 3 - 5 hours. The bacteriocin produced is capable of being adsorbed to a host cell at a pH range of 6 - 6.5 or in a cell-free culture filtrate.